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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
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| - | + | == 22nd July == | |
* Yeast obtained from Dr. Zhao | * Yeast obtained from Dr. Zhao | ||
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| - | + | == 22nd August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
** Result: Failed | ** Result: Failed | ||
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| - | + | == 25th August == | |
* Prepared overnight culture for DNA extraction (3:27pm) | * Prepared overnight culture for DNA extraction (3:27pm) | ||
| - | + | == 26th August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
* Prepped overnight culture | * Prepped overnight culture | ||
| - | + | == 27th August == | |
* Performed PCR (FILL IN PCR TABLE) | * Performed PCR (FILL IN PCR TABLE) | ||
* Prepped 3 overnight cultures | * Prepped 3 overnight cultures | ||
| - | + | == 28th August == | |
* Extracted DNA from 4 cultures | * Extracted DNA from 4 cultures | ||
* Ran gel of PCR products (1.5%, 200V) | * Ran gel of PCR products (1.5%, 200V) | ||
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| - | + | == 2nd September == | |
* FILL IN PCR TABLE | * FILL IN PCR TABLE | ||
| - | + | == 3rd September == | |
* Ran gel | * Ran gel | ||
** Ladder lane 7 | ** Ladder lane 7 | ||
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| - | + | == 8th September == | |
* FILL IN PCR TABLE | * FILL IN PCR TABLE | ||
* Prepped 4 overnight cultures | * Prepped 4 overnight cultures | ||
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| - | + | == 9th September == | |
* Signs of life in 3 of the cultures | * Signs of life in 3 of the cultures | ||
** Wait until tomorrow | ** Wait until tomorrow | ||
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| - | + | == 10th September == | |
* Ran gel again | * Ran gel again | ||
* Split culture | * Split culture | ||
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| - | + | == 11th September == | |
* FILL IN PCR TABLE | * FILL IN PCR TABLE | ||
| - | + | == 12th September == | |
* Isolated DNA from 8 cultures | * Isolated DNA from 8 cultures | ||
* Ran gel | * Ran gel | ||
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| - | + | == 15th September == | |
* PCR PGK Promotor | * PCR PGK Promotor | ||
** Finnzymes Phusion High Fidelity DNA Polymerase | ** Finnzymes Phusion High Fidelity DNA Polymerase | ||
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* second lab procedure | * second lab procedure | ||
** more about second lab procedure | ** more about second lab procedure | ||
| - | COPY/PASTE THEN REMOVE THIS | + | COPY/PASTE THEN REMOVE THIS, BRACKETS, DASHES, AND EXCLAMATION MARK--> |
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Revision as of 01:26, 8 October 2008
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Contents |
22nd July
- Yeast obtained from Dr. Zhao
-- == 24th July ==
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
22nd August
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
25th August
- Prepared overnight culture for DNA extraction (3:27pm)
26th August
- Attempted DNA extraction
- Prepped overnight culture
27th August
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
28th August
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
2nd September
- FILL IN PCR TABLE
3rd September
- Ran gel
- Ladder lane 7
- Sample 7 spilled
- 1% agarose
- Too high
- 120V
- Too low
- 50 minutes
8th September
- FILL IN PCR TABLE
- Prepped 4 overnight cultures
- Yeast dried out again
9th September
- Signs of life in 3 of the cultures
- Wait until tomorrow
- Ran gel on PCR from 8th September
- 150V, 50 minutes
- No sign of DNA
- Ladder from Courtney
10th September
- Ran gel again
- Split culture
- 150V, 50 minutes
- 0.75% gel
- Ladder from Courtney
11th September
- FILL IN PCR TABLE
12th September
- Isolated DNA from 8 cultures
- Ran gel
- 1% agarose
- 150V
- 38 mins
- Poor results
15th September
- PCR PGK Promotor
- Finnzymes Phusion High Fidelity DNA Polymerase
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