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Team:University of Lethbridge/Notebook/Project1October
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| - | Objective: | + | Objective: Create motility media stab tubes controls for motility assay. |
| - | Positive control: K12 ( | + | Positive control: ''E. coli'' K12 (wild-type) |
Negative control: ''Staphylococcus epidermis'' | Negative control: ''Staphylococcus epidermis'' | ||
| - | + | Stabbed motility media (no theophylline) with subcultures of either cell type, using inoculating loops (teeny loop part, essentially a rod). | |
| - | + | ||
| - | + | ||
| + | Incubated for 48 hours at 37 C. | ||
===October 7, 2008=== | ===October 7, 2008=== | ||
Revision as of 03:15, 8 October 2008
Back to The University of Lethbridge Main Notebook
Contents |
October 2, 2008
Munima, Christa, John
Objective: To set up chemotaxis spatial localization assay
Made and autoclaved media to make LB + amp plates to be used in the assay.
Recipe:
- Peptone 5g - Yeast Extract 2.5 g - NaCl 5 g - Agar (0.25 %) 1.25 g - dH2O 500 mL
October 4, 2008
Christa
Objective: To set up chemotaxis spatail locatization assay
Media made on October 2 looked funny.
Poured 23 plates (LB + amp 100ug/mL) anyway. Stored in iGEM 4 C fridge.
October 6, 2008
Christa, Alix
Objective: Make more solutions for future chemical competency experiments.
Made 500 mL of 100 mM CaCl2 solution and 500 mL of 80mM MgCl2-20mM CaCl2 solution. They are stored on the counter under the iGEM glass cabinet in 1 L media bottles.
Selina
Objective: Create motility media stab tubes controls for motility assay.
Positive control: E. coli K12 (wild-type) Negative control: Staphylococcus epidermis
Stabbed motility media (no theophylline) with subcultures of either cell type, using inoculating loops (teeny loop part, essentially a rod).
Incubated for 48 hours at 37 C.
October 7, 2008
Munima, John, Roxanne
Objective: Assess the motility media stab tubes after one day of incubation.
Positive control: K12
Negative control: Staphylococcus epidermis
Side-by-side comparison:
