Team:Warsaw/Calendar-Main/23 June 2008

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<p>Sequencing determined that all white colonies have intact lacZ gene.</p>
 
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<p>Reason of white phenotype unknown.</p>
 
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<p>We suppose that it is due to chromosomal mutation.</p>
 
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<p>We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p>
 
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<h3>PCR results</h3>
<h3>PCR results</h3>
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Still no success. We need to run gradient PCR to find optimal reaction conditions.  
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We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48&deg;C. Mg<sup>2+</sup> concentration elevated to 12 μM.
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Revision as of 16:12, 8 October 2008

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PCR results

We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 12 μM.


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