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Team:Warsaw/Calendar-Main/24 June 2008
From 2008.igem.org
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br> | ||
| - | Template DNA: AID for translation fusion and T7 RNA- | + | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion<br> |
Elongation temperature: 55°C<br> | Elongation temperature: 55°C<br> | ||
Revision as of 16:18, 8 October 2008
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Sequencing determined that all white colonies have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ). PCR resultsStill no success. We need to run gradient PCR to find optimal reaction conditions.Preparation of pMPMT5-AID+AIDT7 construct
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