Team:Chiba/Parts/j

From 2008.igem.org

(Difference between revisions)
(→Reporter)
Line 3: Line 3:
We hope to submit these parts:
We hope to submit these parts:
===Autoinducer synthase===
===Autoinducer synthase===
-
*BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]<Br>lacI promotor regulate lasI gene for an autoinducer synthetase for PAI from Pseudomonas aeruginosa(no LVA).
+
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
-
*BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]<Br>lacI promotor regulate rhlI gene for an autoinducer synthetase for N-butyryl-HSL (BHL) and HHL(no LVA).
+
<tr>
-
*BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]<Br>lacI promotor regulate rhlI gene with LVA.
+
<td width="200">Part Name</td>
-
*BBa_K084010 [http://partsregistry.org/Part:BBa_K084010]<Br>lacI promotor regulate cinI gene with LVA that is for an autoinducer synthetase which utilizes SAM to make the quorum sensing molecule 3OH,C14:1-HSL.
+
<td width="200">Sub Parts</td>
-
*BBa_K084012 [http://partsregistry.org/Part:BBa_K084012]<Br>Generates an untagged LuxI enzyme which produces an AHL(3OC6HSL) under a lac-repressible promoter.
+
<td width="200">Part Size(bp)<td>
-
*BBa_K084014 [http://partsregistry.org/Part:BBa_K084014]<Br>Generates 3OC6-HSL under a lac-repressible promoter
+
<td width="200">Plasmid Vector<td>
 +
<td width="300">Plasmid Size(bp)<td>
 +
<td width="200">Total size(bp)<td>
 +
<td width="200">Resistance<td>
 +
<td width="200">Status<td>
 +
<td width="200">Sequenced<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]</td>
 +
<td width="300">plac+rbs+LasI(no LVA)</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1AK3<td>
 +
<td width="200">3189<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]</td>
 +
<td width="300">plac+rbs+RhlI(no LVA)</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1AK3<td>
 +
<td width="200">3189<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]</td>
 +
<td width="300">plac+rbs+RhlI</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1A2<td>
 +
<td width="200">2079<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084010 [http://partsregistry.org/Part:BBa_K084010]</td>
 +
<td width="300">plac+rbs+CinI</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1A2<td>
 +
<td width="200">2079<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084012 [http://partsregistry.org/Part:BBa_K084012]</td>
 +
<td width="300">plac+rbs+LuxI(no LVA)</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1AK3<td>
 +
<td width="200">3189<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084014 [http://partsregistry.org/Part:BBa_K084014]</td>
 +
<td width="300">plac+rbs+LuxI()</td>
 +
<td width="200"><td>
 +
<td width="200">pSB1A2<td>
 +
<td width="200">2079<td>
 +
<td width="200"><td>
 +
<td width="200">Amp<td>
 +
<td width="350">Glycerol stock in -80/miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
</table>
===Receiver protein===
===Receiver protein===
-
*BBa_K084004 [http://partsregistry.org/Part:BBa_K084004]<Br>Transcriptional regulator RhlR (BBa_C0071) generator regulated by tet promoter
+
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
-
*BBa_K084005 [http://partsregistry.org/Part:BBa_K084005]<Br>Transcriptional regulator LasR (BBa_C0079) generator regulated by tet promoter
+
<tr>
-
*BBa_K084006 [http://partsregistry.org/Part:BBa_K084006]<Br>Transcriptional regulator CinR (BBa_C0077) generator regulated by tet promoter
+
<td width="200">Part Name</td>
 +
<td width="200">Sub Parts</td>
 +
<td width="200">Part Size(bp)<td>
 +
<td width="200">Plasmid Vector<td>
 +
<td width="300">Plasmid Size(bp)<td>
 +
<td width="200">Total size(bp)<td>
 +
<td width="200">Resistance<td>
 +
<td width="200">Status<td>
 +
<td width="200">Sequenced<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084004 [http://partsregistry.org/Part:BBa_K084004]</td>
 +
<td width="300">ptet+rbs+RhlR</td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="350">miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084005 [http://partsregistry.org/Part:BBa_K084005]</td>
 +
<td width="300">ptet+rbs+LasR</td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="350">miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
<tr>
 +
<td width="200">BBa_K084006 [http://partsregistry.org/Part:BBa_K084006]</td>
 +
<td width="300">ptet+rbs+CinRI()</td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="200"><td>
 +
<td width="350">miniprepped<td>
 +
<td width="50">ok<td>
 +
</tr>
 +
</table>
===Reporter===
===Reporter===

Revision as of 09:02, 9 October 2008

> ホーム

We hope to submit these parts:

Autoinducer synthase

Part Name Sub Parts Part Size(bp) Plasmid Vector Plasmid Size(bp) Total size(bp) Resistance Status Sequenced
BBa_K084007 [http://partsregistry.org/Part:BBa_K084007] plac+rbs+LasI(no LVA) pSB1AK3 3189 Amp Glycerol stock in -80/miniprepped ok
BBa_K084008 [http://partsregistry.org/Part:BBa_K084008] plac+rbs+RhlI(no LVA) pSB1AK3 3189 Amp Glycerol stock in -80/miniprepped ok
BBa_K084009 [http://partsregistry.org/Part:BBa_K084009] plac+rbs+RhlI pSB1A2 2079 Amp Glycerol stock in -80/miniprepped ok
BBa_K084010 [http://partsregistry.org/Part:BBa_K084010] plac+rbs+CinI pSB1A2 2079 Amp Glycerol stock in -80/miniprepped ok
BBa_K084012 [http://partsregistry.org/Part:BBa_K084012] plac+rbs+LuxI(no LVA) pSB1AK3 3189 Amp Glycerol stock in -80/miniprepped ok
BBa_K084014 [http://partsregistry.org/Part:BBa_K084014] plac+rbs+LuxI() pSB1A2 2079 Amp Glycerol stock in -80/miniprepped ok

Receiver protein

Part Name Sub Parts Part Size(bp) Plasmid Vector Plasmid Size(bp) Total size(bp) Resistance Status Sequenced
BBa_K084004 [http://partsregistry.org/Part:BBa_K084004] ptet+rbs+RhlR miniprepped ok
BBa_K084005 [http://partsregistry.org/Part:BBa_K084005] ptet+rbs+LasR miniprepped ok
BBa_K084006 [http://partsregistry.org/Part:BBa_K084006] ptet+rbs+CinRI() miniprepped ok

Reporter

  • BBa_K084003 [http://partsregistry.org/Part:BBa_K084003]
    Venus YFP Producer Controlled by 3OC6HSL Receiver


ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/Parts/j"