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Team:Warsaw/Calendar-Main/28 July 2008
From 2008.igem.org
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| - | <ol><li>Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr). </li> | + | <ol><li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#digest">Digest</a> of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr). </li> |
| - | <li>Gel elctrophoresis and gel-out of proper band - 4300 bp. </li> | + | <li>Gel elctrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band - 4300 bp. </li> |
| - | <li>Ligation of isolated DNA fragment (1 hr). </li> | + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment (1 hr). </li> |
| - | <li>Transformation of E. coli TOP10 strain with ligation. </li> | + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a>of E. coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li> |
<li>Transformants plating on LB + kanamycin. </li></ol> | <li>Transformants plating on LB + kanamycin. </li></ol> | ||
Revision as of 13:10, 9 October 2008
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Sprawdzanie czy omp_omega_A_alfa daje oporność na AP
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