Team:Warsaw/Calendar-Main/30 July 2008
From 2008.igem.org
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<tr><th>100</th><td class="live">0.02</td><td class="live">0,396</td><td class="live">0.563</td><td class="live">0.678</td><td class="live">0.602</td><td class="live">0.611</td></tr> | <tr><th>100</th><td class="live">0.02</td><td class="live">0,396</td><td class="live">0.563</td><td class="live">0.678</td><td class="live">0.602</td><td class="live">0.611</td></tr> | ||
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+ | Wniosek: najlepsz indukcja jest 0,5 - 0,25 mM IPTG, a ampicyliny dawać należy 50 - 75 mikrograma na mililitr Ampicyliuny | ||
Revision as of 14:11, 9 October 2008
1. Isolation of plasmids from cultures inocluated on previous day. Checking if OmpA_omega_A_alpha gives ampicillin resistance Inoculation of OmpA_omega_A_alpha from various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 into the same IPTG concentrations, but with various ampicillin concentrations (25, 50, 75, 100) in relation 1:50 Bacterial growth (OD) measurement in the evening:
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