Team:Warsaw/Calendar-Main/31 July 2008
From 2008.igem.org
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7. Overnight ligation: pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA. | 7. Overnight ligation: pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA. | ||
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- | Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt) | + | |
+ | <center><h4>Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)</center>Piotr:</h4> | ||
Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5IPTG) in <i>E. coli</i> Rosetta strain. | Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5IPTG) in <i>E. coli</i> Rosetta strain. |
Revision as of 14:35, 9 October 2008
1. Optimization of PCR to obtain truncated fragment of protein A DNA Elongation time: 30s Annealing temperature: 60°C 20 cycles 3. Gel electrophoresis and isolation of 250 bp band.
Checking if degradation of fusion with OmpA is a result of Top10 proteases' activity (lon, iompt)</center>Piotr:Inoculation of OmpA_omega_A_alpha and OmpA_A_alpha with and without inductor (0,5IPTG) in E. coli Rosetta strain.
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