Team:Valencia/prueba3

From 2008.igem.org

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introduction to Synthetic Biology 2008
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Introduction to Synthetic Biology 2008
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<h1><b>Main Project</b> - [http://parts.mit.edu/igem07/index.php/Imperial/Infector_Detector/Introduction Infector Detector]</h1>  
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<h1><b>Main Project</b> - [https://2007.igem.org/Imperial/Infector_Detector/Introduction Infector Detector]</h1>  
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Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. ([http://parts.mit.edu/igem07/index.php/Imperial/Infector_Detector/Introduction more...])
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Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.<i>coli</i>, and output a fluorescent protein as a result. ([https://2007.igem.org/Imperial/Infector_Detector/Introduction more...])
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                       <li>Successfully utilized <b>cell-free chassis</b> for Infector Detector
                       <li>Successfully utilized <b>cell-free chassis</b> for Infector Detector
                       <li>Characterised Infector Detector to <b>detect AHL at ambient temperatures</b>
                       <li>Characterised Infector Detector to <b>detect AHL at ambient temperatures</b>
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                       <li><a href="http://parts.mit.edu/igem07/index.php/Imperial/Infector_Detector/F2620_Comparison"><b>Compared system expression</b></a> to <a href="http://parts.mit.edu/registry/index.php/Part:BBa_F2620">AHL Receiver (BBa_F2620)</a> transfer function.
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                       <li><a href="https://2007.igem.org/Imperial/Infector_Detector/F2620_Comparison"><b>Compared system expression</b></a> to <a href="http://partsregistry.org/Part:BBa_F2620">AHL Receiver (BBa_F2620)</a> transfer function.
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{|border="1"
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|-
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|width="300px"|'''Specification'''
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|width="420px"|'''Design Solution'''
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|width="100px"|'''System Level'''
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|- style="background:#eeffee"
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|'''Health & Safety''' - System must not contain living cells, or cause any harm to patient.
 +
|Use a Cell Free System e.g. Promega's S30 Cell Extract
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|Chassis
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|- style="background:#eeffee"
 +
|'''Shelf-life''' - System must have a shelf life of 7 days.
 +
|Use a Cell Free System that can be packaged and stored in a -80&deg;C freezer for 7 days.
 +
|Chassis
 +
|- style="background:#eeffee"
 +
|'''Packaging and Application''' - System must be applied as a cream or spray.
 +
|Package the system in liquid form.
 +
|Chassis
 +
|- style="background:#eeeeff"
 +
|'''Inputs''' - System must be sensitive to AHL concentration between 5-50nM.
 +
|Sensitive AHL receiver to detect low AHL levels - Part no. F2620.
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|Construct
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|- style="background:#eeeeff"
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|'''Outputs''' - System must give a visual signal if AHL is present.
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|Couple AHL to a reporter system expressing fluoresent protein eg. RFP
 +
|Construct
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|- style="background:#ffeeee"
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|'''Operating Conditions''' - System must operate within temperature 20&deg;-30&deg;C.
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|Gene expression systems and protein must be thermostable. Characterise a Cell Free System at these temperatures.
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|Construct & Chassis
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|- style="background:#ffeeee"
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|'''Response Time''' - System needs to have a response time under 3 hours.
 +
|To be Determined - this is hard to design for. Protease Inhibitor of Cell Extract should ensure degradation of Visual Reporter is minimal.
 +
|Construct & Chassis
 +
|}
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 +
 +
 +
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{| style="width:800px;" cellpadding="2" cellspacing="0" align="center" border="1"
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|-
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|style="background:#ffffcc"|<center>Properties</center>||<center>'''Rabbit Reticulocyte Lysate'''</center>||<center>'''Wheat Germ Extract'''</center>||<center>'''''E. coli'' Extract'''</center>||<center>'''Reconstituted Extract'''</center>
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|-
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|style="background:#ffffcc"|<center>Types</center>||<center>Eukaryotic</center>||<center>Eukaryotic</center>||<center>Prokaryotic</center>||<center>Artificial</center>
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|-
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|style="background:#ffffcc"|<center>Uses</center>||<center>Widely used for in-vitro translation</center>||<center>Mostly used for in-vitro translation</center>||<center>Mostly used for coupled transcription-translation</center>||<center>Used for in-vitro translation</center>
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|-
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|style="background:#ffffcc"|<center>Templates</center>||
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*mRNAs from viruses or eukaryotes
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*Capped or un-capped mRNAs
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||
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*mRNAs from viruses or eukaryotes
 +
*Capped mRNAs
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||
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*Viral mRNAs with stable secondary structures
 +
*Un-capped mRNAs
 +
*Plasmid or linear DNAs
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||
 +
*Capped or uncapped mRNAs
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|-
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|style="background:#ffffcc"|<center>Post-translational modifications</center>||<center>Yes</center>||<center>Yes</center>||<center>No</center>||<center>Yes</center>
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|}

Latest revision as of 15:06, 9 October 2008

Contents

Super titulo

Hola iGEMITOS

Probando




Home The Team The Project Parts Submitted to the Registry Modeling Notebook
Home The Team The Project Parts Submitted to the Registry Modeling Notebook


introduction to Synthetic Biology 2008


Introduction to Synthetic Biology 2008


Hot (and sexy) Yeast

Have you ever forgotten to keep your culture on the stove? Isn´t it annoying to lose several week's work? Tired of apologizing to your boss? WE HAVE THE SOLUTION!!! Valencia iGEM '08 Team heat it up in your lab!!! Besetes en el culete! --- No asustarse, estamos probando colores ---


Main Project - Infector Detector

Infector Detector is a system that will detect the presence of biofilm infections on urinary catheters by reporting on the presence of AHL, a signalling molecule used by E.coli, and output a fluorescent protein as a result. (more...)

MIT Teachers' workshop

Main Achievements

  • Designed and modelled a device to detect biofilms and prevent urinary catheter infections like MRSA
  • Successfully utilized cell-free chassis for Infector Detector
  • Characterised Infector Detector to detect AHL at ambient temperatures
  • Compared system expression to AHL Receiver (BBa_F2620) transfer function.

  • Discount DNA Synthesis from Geneart. iGEM's DNA synthesis partner offers iGEM teams highly discounted DNA synthesis: $0.20 per base and $35 shipping. Check out the resources box for more information.
  • Are you having trouble transforming your DNA from the 2008 Spring DNA Distribution? Please stay tuned. We have sent out a hard copy of the first volume of out iGEM Newsletter which has information discussing this with tips on how to get better results. Download the online pdf version of the newsletter here.


Jaume quiere saber si se desmonta
Meri
Joaquina
4
5
6
7
8








iGEM Chiba 2008 Team Wiki


Welcome!

Esto va dentro de la tabla welcome???
















Members


  • Takayoshi Kubo
  • Yoshimi Iyama
  • Kohei Kawasaki
  • Takahiro Katori
  • Masahiro Tominaga
  • Hiroki Fukutomi
  • Mai Sugiyama
  • Aoi Kobayashi









Specification Design Solution System Level
Health & Safety - System must not contain living cells, or cause any harm to patient. Use a Cell Free System e.g. Promega's S30 Cell Extract Chassis
Shelf-life - System must have a shelf life of 7 days. Use a Cell Free System that can be packaged and stored in a -80°C freezer for 7 days. Chassis
Packaging and Application - System must be applied as a cream or spray. Package the system in liquid form. Chassis
Inputs - System must be sensitive to AHL concentration between 5-50nM. Sensitive AHL receiver to detect low AHL levels - Part no. F2620. Construct
Outputs - System must give a visual signal if AHL is present. Couple AHL to a reporter system expressing fluoresent protein eg. RFP Construct
Operating Conditions - System must operate within temperature 20°-30°C. Gene expression systems and protein must be thermostable. Characterise a Cell Free System at these temperatures. Construct & Chassis
Response Time - System needs to have a response time under 3 hours. To be Determined - this is hard to design for. Protease Inhibitor of Cell Extract should ensure degradation of Visual Reporter is minimal. Construct & Chassis



Properties
Rabbit Reticulocyte Lysate
Wheat Germ Extract
E. coli Extract
Reconstituted Extract
Types
Eukaryotic
Eukaryotic
Prokaryotic
Artificial
Uses
Widely used for in-vitro translation
Mostly used for in-vitro translation
Mostly used for coupled transcription-translation
Used for in-vitro translation
Templates
  • mRNAs from viruses or eukaryotes
  • Capped or un-capped mRNAs
  • mRNAs from viruses or eukaryotes
  • Capped mRNAs
  • Viral mRNAs with stable secondary structures
  • Un-capped mRNAs
  • Plasmid or linear DNAs
  • Capped or uncapped mRNAs
Post-translational modifications
Yes
Yes
No
Yes



  Links
Brown iGem on Openwetware
Brown iGem Official Website
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