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Team:Warsaw/Calendar-Main/23 June 2008
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| - | + | <h3>Preparation of constructs with OmpA protein fusions<br> | |
| - | <h3>Preparation of constructs with OmpA protein fusions< | + | Michał K.</h3> |
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<li> Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</li> | <li> Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</li> | ||
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| + | <li> PCR on isolated plasmids with [???]</li></ol> | ||
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| + | <h3>PCR results<br> | ||
| + | Michał L., Ewa, Marcin</h3> | ||
| + | <p>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 12 μM.</p> | ||
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Revision as of 15:25, 9 October 2008
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Preparation of constructs with OmpA protein fusions
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