Team:Warsaw/Calendar-Main/21 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
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<h4>PCR</h4>
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<table id="result">
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<tr><th>Product</th><th>Template</th><th>Primers</th><th>Product length</th></tr>
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<tr><th>linker-A</th><td>pDRIVE-TapTag</td><td>AL+link10+homo2 + AP+NotI </td><td>470 bp</td></tr>
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<tr><th>alpha-linker</th><td>pUC19</td><td>AlphaL+SacI + AlphaP+link10+homo2 </td><td>1022 bp</td></tr>
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<tr><th>omega-linker</th><td>pUC19</td><td>OmegaL+SacI + OmegaP+link10+homo2 </td><td>779 bp</td></tr>
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</table>
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<h4>Universal PCR program for A, alpha and omega</h4>
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<table id="result">
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<tr><th>Temperature</th><th>Time</th></tr>
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<tr><td>94&deg;C</td><td>4:00</td></tr>
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<tr><td>94&deg;C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr>
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<tr><td>50&deg;C</td><td>0:45</td></tr>
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<tr><td>72&deg;C</td><td>2:00</td></tr>
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<tr><td>72&deg;C</td><td>10:00</td></tr>
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<tr><td>4&deg;C</td><td>infinite</td></tr>
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</table>
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<p>Polymerase: homemade Pfu-Turbo</p>
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Latest revision as of 18:17, 9 October 2008

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