Team:Warsaw/Calendar-Main/17 June 2008

From 2008.igem.org

(Difference between revisions)
Line 24: Line 24:
<li> Inoculation of  <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with pACYC177 plasmid into liquid LB + kanamycin. </li>  
<li> Inoculation of  <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with pACYC177 plasmid into liquid LB + kanamycin. </li>  
</ol></p>
</ol></p>
-
 
+
<h3> Blue/white and rifampicin test </h3>
<h4>Michał L., Ewa, Marcin:</h4>
<h4>Michał L., Ewa, Marcin:</h4>
<p>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></p>
<p>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></p>

Revision as of 20:37, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of constructs with OmpA protein fusions
Michał K.

  1. PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
  2. PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
  3. Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega.
  4. Electrophoresis to estimate the concentration of isolated DNA.
  5. Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.
  6. Inoculation of E. coli TOP10 with pACYC177 plasmid into liquid LB + kanamycin.

Blue/white and rifampicin test

Michał L., Ewa, Marcin:

Repetition of experiment from 11/06/2008.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/17_June_2008"