Preparation of alpa-A and omega-A fusions
Michał L., Ewa, Marcin

Preparation of constructs with OmpA protein fusions
Michał K.

1. PCR on isolated plasmids with OmpaL_N and OmpaP_link primers
2. Gel electrophoresis and DNA isolation from proper band (obtained with 20 cycles)

Blue/white and rifampicin test
Michał L., Ewa, Marcin

Sequencing determined that all white colonies from 16 June have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).