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Team:Warsaw/Calendar-Main/25 June 2008
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<li>We've ran gradient <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain Alpha-link and link-A. <br>Annealing temp 48°C - 55°C.<br> | <li>We've ran gradient <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain Alpha-link and link-A. <br>Annealing temp 48°C - 55°C.<br> | ||
| - | Elongation time 1 - 2 | + | Elongation time 1 - 2 minutes.</li> |
<li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A. </li></ol> | <li>We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A. </li></ol> | ||
Revision as of 12:34, 10 October 2008
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Preparation of constructs with OmpA protein fusions
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