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Team:Warsaw/Calendar-Main/5 July 2008
From 2008.igem.org
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| - | < | + | <h3>Cloning omega-A fusion on pKS (second attempt)</h3> |
| + | <h4>Michał L., Ewa, Marcin:</h4> | ||
| + | <p>We have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:</p><br/> | ||
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| + | <table id="result"> | ||
| + | <tr ><th colspan="4"><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a></td></tr> | ||
| + | <tr><th>Product</th><th>Template</th><th>Primers</th><th>Product length</th></tr> | ||
| + | <tr><th>linker-A</th><td>pDRIVE-TapTag</td><td><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+link10+homo2">AL+link10+homo2</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> </td><td>470 bp</td></tr> | ||
| + | <tr><th>omega-linker</th><td>pUC19</td><td><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL+SacI">OmegaL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP+link10+homo2">OmegaP+link10+homo2</a></td><td>400 bp</td></tr> | ||
| + | </table> | ||
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| + | <h4 style="test-align: center">PCR program for linker-A and omega-linker</h4> | ||
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| + | <table id="result"> | ||
| + | <tr><th>Temperature</th><th>Time</th></tr> | ||
| + | <tr><td>94°C</td><td>4:00</td></tr> | ||
| + | <tr><td>94°C</td><td>0:30</td><td rowspan="3">28 cycles</td></tr> | ||
| + | <tr><td>gradient 48-55°C</td><td>0:45</td></tr> | ||
| + | <tr><td>72°C</td><td>0:50</td></tr> | ||
| + | <tr><td>72°C</td><td>10:00</td></tr> | ||
| + | <tr><td>4°C</td><td>infinite</td></tr> | ||
| + | </table> | ||
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Revision as of 13:48, 10 October 2008
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Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin:We have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:
PCR program for linker-A and omega-linker
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