Team:Warsaw/Calendar-Main/6 July 2008

From 2008.igem.org

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<li>Gel electrophoresis of PCR products</li>
<li>Gel electrophoresis of PCR products</li>
<li>Gel-out of proper bands</li>
<li>Gel-out of proper bands</li>
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<li>PCL reaction to create omega-A fusion:</li>
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<li>PCL reaction to create omega-A fusion:<br>
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<table id="result">
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<tr><td>4&deg;C</td><td>infinite</td></tr>
<tr><td>4&deg;C</td><td>infinite</td></tr>
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</li>
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<li>Gel electrophoresis of PCL products</li>
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<li>We have obtained no PCL product (weird?)</li>
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</ol>
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Revision as of 14:02, 10 October 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

  1. Gel electrophoresis of PCR products
  2. Gel-out of proper bands
  3. PCL reaction to create omega-A fusion:

    ProductTemplatesPrimersProduct length
    Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 1440 bp

    PCL program for omega-A fusion

    TemperatureTime
    94°C4:00
    94°C0:3028 cycles
    48°C-58°C0:45
    68°C2:00
    68°C10:00
    4°Cinfinite
  4. Gel electrophoresis of PCL products
  5. We have obtained no PCL product (weird?)