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Team:Warsaw/Calendar-Main/14 May 2008
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<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
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| - | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</li> | + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day. </li> |
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| - | <li> Gel electrophoresis - choice of proper clones (all checked colonies). </li> | + | <li> Gel electrophoresis - choice of proper clones (all checked colonies). Fig. 1.</li> |
<img src="https://static.igem.org/mediawiki/2008/e/e7/Ligation_test_WAW.jpg" width=350/> | <img src="https://static.igem.org/mediawiki/2008/e/e7/Ligation_test_WAW.jpg" width=350/> | ||
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<li> Optimization of conditions for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C); Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematic PCR). <br> | <li> Optimization of conditions for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C); Taq polymerase with supplemented buffer (Łukasz Banasiak helped us with that problematic PCR). <br> | ||
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<li> Gel electrophoresis of PCR products. </li> | <li> Gel electrophoresis of PCR products. </li> | ||
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| + | <var>Fig. 1. 1-DNA ladder; 2 to 9-plasmids isolated from 8 colonies of transformants and digested with HindIII and NcoI</var> | ||
<img src="https://static.igem.org/mediawiki/2008/6/6c/T7grad_WAW2.jpg" width=350/> | <img src="https://static.igem.org/mediawiki/2008/6/6c/T7grad_WAW2.jpg" width=350/> | ||
<var>PCR products. 2-annealing temperature 62°C, 6-annealing temperature 82°C</var> | <var>PCR products. 2-annealing temperature 62°C, 6-annealing temperature 82°C</var> | ||
Revision as of 09:09, 11 October 2008
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Preparation of pMPMT5+AID construct and PCRs for fusionsMichał K.
PCR products. 2-annealing temperature 62°C, 6-annealing temperature 82°C
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