Team:Warsaw/Calendar-Main/17 June 2008

(Difference between revisions)

Revision as of 10:54, 11 October 2008


	Attribution analyzer / Interactive list of topics

PCR on OmpA_linker and linker_alpha with OmpaL_N and AlphaP_XB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
PCR on OmpA_linker and linker_omega with OmpaL_N and OmegaP_EPB primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57°C).
Gel electrophoresis of PCR products and gel-out of OmpA_alpha (900 bp) and OmpA_omega (700 bp).
Electrophoresis to estimate the concentration of isolated DNA.
Overnight digest of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI.
Inoculation of E. coli TOP10 with pACYC177 plasmid into liquid LB + kanamycin.

Repetition of experiment from 11/06/2008.

@@ Line 19: / Line 19: @@
   primers (20 cycles, elongation length 1 min 15 s, annealing temperature 57&deg;C).</li>
 <li> Gel electrophoresis of PCR products and
-<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of OmpA_alpha and OmpA_omega. </li>
+<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of OmpA_alpha (900 bp) and OmpA_omega (700 bp). </li>
 <li>Electrophoresis to estimate the concentration of isolated DNA.</li>
 <li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI. </li>
@@ Line 25: / Line 25: @@
 </ol></p>
 <h3> Blue/white and rifampicin test </h3>
-<h4>Michał L., Ewa, Marcin:</h4>
+<h4>Michał L., Ewa, Marcin</h4>
 <p>Repetition of experiment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/11_June_2008">11/06/2008.</a></p>