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Team:Warsaw/Calendar-Main/24 June 2008
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| - | <h3>Preparation of alpha-A and omega-A fusions< | + | <h3>Preparation of alpha-A and omega-A fusions</h3> |
| - | Michał L., Ewa, Marcin</ | + | <h4>Michał L., Ewa, Marcin</h4> |
| - | Still no success. We need to run gradient PCR to find optimal reaction conditions. | + | <p>Still no success. We need to run gradient PCR to find optimal reaction conditions.</p> |
| - | <h3>Preparation of constructs with OmpA protein fusions< | + | <h3>Preparation of constructs with OmpA protein fusions</h3> |
| - | Michał K.</h3> | + | <h4>Michał K.</h3> |
<ol><li> PCR on isolated plasmids with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <ol><li> PCR on isolated plasmids with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
primers.</li> | primers.</li> | ||
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</ol> | </ol> | ||
| - | <h3> Blue/white and rifampicin test < | + | <h3> Blue/white and rifampicin test</h3> |
| - | Michał L., Ewa, Marcin</h3> | + | <h4>Michał L., Ewa, Marcin</h3> |
<p>Sequencing determined that all white colonies from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a> have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p> | <p>Sequencing determined that all white colonies from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a> have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p> | ||
Revision as of 11:21, 11 October 2008
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Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinStill no success. We need to run gradient PCR to find optimal reaction conditions. Preparation of constructs with OmpA protein fusionsMichał K.
Sequencing determined that all white colonies from 16 June have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).
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