Team:Warsaw/Calendar-Main/25 June 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
<html>
<html>
-
<h3>Preparation of constructs with OmpA protein fusions<br>
+
<h3>Preparation of constructs with OmpA protein fusions</h3>
-
Michał K.</h3>
+
<h4>Michał K.</h4>
-
<p>
+
<p>Repetition of PCRs and gel-out isolations from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June.</a>
-
<ol>
+
-
<li> Repetition of PCRs and gel-out isolations from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June.</a></li></ol>
+
</p>
</p>
-
<h3>PCR of Alpha-link and link-A<br>
+
<h3>PCR of Alpha-link and link-A</h3>
-
Michał L. Marcin and Ewa</h3>
+
<h4>Michał L. Marcin and Ewa</h4>
<ol>
<ol>
<li>We've ran gradient <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain Alpha-link and link-A. <br>Annealing temp 48&deg;C - 55&deg;C.<br>
<li>We've ran gradient <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> to obtain Alpha-link and link-A. <br>Annealing temp 48&deg;C - 55&deg;C.<br>

Revision as of 11:24, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs and gel-out isolations from 16 June.

PCR of Alpha-link and link-A

Michał L. Marcin and Ewa

  1. We've ran gradient PCR to obtain Alpha-link and link-A.
    Annealing temp 48°C - 55°C.
    Elongation time 1 - 2 minutes.
  2. We have successfully amplified Alpha-link and link-A. Now its time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/25_June_2008"