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Team:Warsaw/Calendar-Main/7 July 2008
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<h3>Preparation of constructs with OmpA protein fusions</h3> | <h3>Preparation of constructs with OmpA protein fusions</h3> | ||
| - | <h4>Piotr | + | <h4>Piotr</h4> |
<p> | <p> | ||
<ol> | <ol> | ||
| - | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI. </li> | + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of confirmed clones of pET15b+OmpA_alpha and pET15b+OmpA_omega with NdeI and BamHI (Tango 2x buffer). </li> |
<li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pACYC177 plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>. </li> | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pACYC177 plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>. </li> | ||
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (OmpA_alpha- 1000 bp, OmpA_omega - 800 bp). </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (OmpA_alpha- 1000 bp, OmpA_omega - 800 bp). </li> | ||
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<h3>Cloning omega-A fusion on pKS (second attempt)</h3> | <h3>Cloning omega-A fusion on pKS (second attempt)</h3> | ||
| - | <h4>Michał L., Ewa, Marcin | + | <h4>Michał L., Ewa, Marcin</h4> |
<p>We have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:</p><br/> | <p>We have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:</p><br/> | ||
Revision as of 14:25, 11 October 2008
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Preparation of constructs with OmpA protein fusionsPiotr
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, MarcinWe have just received corrected version of omegaP-link10-homo2 primer. Let's hope this time everything will be fine. We are repeating the PCRs:
PCR program for linker-A and omega-linker
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