"
Team:Warsaw/Calendar-Main/8 July 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
| Line 18: | Line 18: | ||
<ol> | <ol> | ||
<li>Gel electrophoresis of PCR products.</li> | <li>Gel electrophoresis of PCR products.</li> | ||
| - | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper band ( | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of proper band (900 bp).</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">PCL</a> reaction to create omega-A fusion:<br> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcl">PCL</a> reaction to create omega-A fusion:<br> | ||
| Line 25: | Line 25: | ||
<table id="result"> | <table id="result"> | ||
<tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr> | <tr><th>Product</th><th>Templates</th><th>Primers</th><th>Product length</th></tr> | ||
| - | <tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td> | + | <tr><th>Omega-A fusion</th><td>Omega-linker + linker-A</td><td>OmegaL+SacI + AP+NotI </td><td>900 bp</td></tr> |
</table> | </table> | ||
<br> | <br> | ||
Revision as of 14:42, 11 October 2008
 |
|
||||||||||||||||||||||||||||||||||
 |
|
||||||||||||||||||||||||||||||||||
Preparation of constructs with OmpA protein fusionsPiotr
Cloning omega-A fusion on pKS (second attempt)Michał L., Ewa, Marcin
|
|
||||||||||||||||||||||||||||||||||
