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Team:Warsaw/Calendar-Main/10 July 2008
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<h3>Preparation of constructs with OmpA protein fusions</h3> | <h3>Preparation of constructs with OmpA protein fusions</h3> | ||
| - | <p><ol> | + | <h4> Michał K.</h4><p><ol> |
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day. </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day. </li> | ||
| - | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and NotI (we confirmed pCACYC177 + OmpA_omega | + | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and NotI (BamHI buffer) - we confirmed pCACYC177 + OmpA_omega. We didn't obtain pCACYC177 + OmpA_alpha probably because of a mistake in plating. </li> |
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pACYC177 and OmpA_alpha (1 hr)</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pACYC177 and OmpA_alpha (1 hr).</li> |
<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products: pACYC177 and OmpA_alpha.</li> | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products: pACYC177 and OmpA_alpha.</li> | ||
<li> Transformants plating on LB + kanamycin.</li> | <li> Transformants plating on LB + kanamycin.</li> | ||
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| - | <h3>Preparation of construct pKS with A protein< | + | <h3>Preparation of construct pKS with A protein</h3> |
| - | Michał L., Marcin:</ | + | <h4>Michał L., Marcin:</h4> |
<p><ol> | <p><ol> | ||
<li> Inactivation of digestion enzymes and CIAP.</li> | <li> Inactivation of digestion enzymes and CIAP.</li> | ||
Revision as of 14:53, 11 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Marcin:
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