Team:Warsaw/Calendar-Main/19 May 2008

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Revision as of 15:17, 11 October 2008


	Attribution analyzer / Interactive list of topics

PCRs for fusions

Michał K.

PCR - translational fusion: AID + T7 RNA-polymerase
Primers:
- AIDlNrH
- T7pXbSal
Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translational fusion
Annealing temperature : 73°C
Elongation time: 4 minutes
20 cycles
Gel electrophoresis and isolation of proper band (3300 bp). Fig. 1.
Electrophoresis to estimate the concentration of isolated DNA.

Rifampicin Test

Michał L., Ewa, Marcin

Overnight inoculation and induction:
Top10 + 0.5 mM IPTG
pMPM-AID + Glc + 0.01% L-Ara
pTrc99a-AID + Glc + 0.5 mM IPTG
Fig. 1. 1-DNA ladder; 2-PCR product-translational fusion

@@ Line 5: / Line 5: @@
 <p>
 <ol>
-<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase<br>
+<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translational fusion: AID + T7 RNA-polymerase<br>
 Primers: <br>
@@ Line 16: / Line 16: @@
-Template DNA: purified PCR products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> - AID and T7 RNA-polymerase for translation fusion <br>
+Template DNA: purified PCR products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> - AID and T7 RNA-polymerase for translational fusion <br>
 Annealing temperature : 73&deg;C <br>
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 <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a> + Glc + 0.5 mM IPTG<br>
 <img src="https://static.igem.org/mediawiki/2008/e/e5/Pcr_fusion_WAW.jpg" width=300/>
-<var>Fig. 1. 1-DNA ladder; 2-PCR product-translation fusion</var></html>
+<var><b>Fig. 1.</b> 1-DNA ladder; 2-PCR product-translational fusion</var></html>
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