Team:Warsaw/Calendar-Main/21 May 2008

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(Difference between revisions)
Line 17: Line 17:
<tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
<tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
<tr><th>Top10</th><td>none</td><td>12</td></tr>
<tr><th>Top10</th><td>none</td><td>12</td></tr>
-
<tr><th>Top10</th><td>10mM IPTG</td><td>17</td></tr>
+
<tr><th>Top10</th><td>1mM IPTG</td><td>17</td></tr>
<tr><th>Top10 pMPM-AID</th><td>none</td><td>13</td></tr>
<tr><th>Top10 pMPM-AID</th><td>none</td><td>13</td></tr>
-
<tr><th>Top10 pMPM-AID</th><td>1% Glucose</td><td>18</td></tr>
+
<tr><th>Top10 pMPM-AID</th><td>0.1% Glucose</td><td>18</td></tr>
<tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>54</td></tr>
<tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>54</td></tr>
<tr><th>Top10 pTRC-AID</th><td>none</td><td>14</td></tr>
<tr><th>Top10 pTRC-AID</th><td>none</td><td>14</td></tr>

Revision as of 16:20, 11 October 2008

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Michał K.

  1. Inoculation of separate transformant colonies for transcription fusion (liquid LB + tetracycline).
  2. Inoculation of separate transformant colonies for translation fusion (liquid LB + tetracycline).

Results of rifampicin test

Michał L., Ewa, Marcin

StrainInducer / repressorNumber of colonies on rifampicin
Top10none12
Top101mM IPTG17
Top10 pMPM-AIDnone13
Top10 pMPM-AID0.1% Glucose18
Top10 pMPM-AID0.1% Arabinose54
Top10 pTRC-AIDnone14
Top10 pTRC-AID1% Glucose17
Top10 pTRC-AID0.1% Arabinose53

Conclusion: AID seems to work!

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