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Team:ETH Zurich/Overview
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''''''Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome''''''<br> | ''''''Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome''''''<br> | ||
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This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome. | This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome. | ||
Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering. | Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering. | ||
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We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection .<br> | We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection .<br> | ||
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==="Meet us" video=== | ==="Meet us" video=== | ||
Revision as of 16:22, 11 October 2008
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Project Abstract'Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome' This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome.
Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering.
We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is base on an iterative cycle of genome reduction and strain selection.
We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection . "Meet us" video
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