Team:Warsaw/Calendar-Main/18 June 2008

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Revision as of 16:24, 11 October 2008


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Isolation of pACYC177.
Digest of purified pACYC177 with NdeI and BamHI (Tango 2x buffer).
Gel electrophoresis and isolation of proper band (3200 bp).
Clean-up of OmpA_alpha and OmpA_omega digest products.
Ligation of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).
Transformation of E.coli TOP10 with ligation products.
Plating transformants on LB+tetracycline.

Counting of blue colonies:

Results of rifampicin test
Strain	Inducer / repressor	Number of colonies on rifampicin
Top10	none	8
Top10 pMPM-AID	0.1% Arabinose	72
Top10 pMPM-AID	none	4
Top10 pMPM-A+T13	0.1% Arabinose	148
Top10 pMPM-A+T13	none	6
Top10 pMPM-AT6	0.1% Arabinose	21
Top10 pMPM-AT6	none	27

@@ Line 33: / Line 33: @@
 <tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>72</td></tr>
 <tr><th>Top10 pMPM-AID</th><td>none</td><td>4</td></tr>
-<tr><th>Top10 pMPM-A+T13</th><td>0.1% Arabinose</td><td>21</td></tr>
+<tr><th>Top10 pMPM-A+T13</th><td>0.1% Arabinose</td><td>148</td></tr>
 <tr><th>Top10 pMPM-A+T13</th><td>none</td><td>6</td></tr>
-<tr><th>Top10 pMPM-AT6</th><td>0.1% Arabinose</td><td>148</td></tr>
+<tr><th>Top10 pMPM-AT6</th><td>0.1% Arabinose</td><td>21</td></tr>
 <tr><th>Top10 pMPM-AT6</th><td>none</td><td>27</td></tr>
 </table>