Team:Warsaw/Calendar-Main/17 July 2008

(Difference between revisions)

Revision as of 17:27, 11 October 2008


	Attribution analyzer / Interactive list of topics

Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI (BamHI buffer).
Gel eloctrophoresis and gel-out (4300 bp band).
Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
Transformation of E. coli TOP10 strain with ligation.
Transformants plating on LB + kanamycin.

@@ Line 1: / Line 1: @@
 {{WarNotebook}}
 <!-- do not edit above me! -->
 <html>
+<h3>Cloning of protein Z DNA to OmpA constructs</h3><h4>Michał K.</h4>
-<h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3>
 <p><ol>
 <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI (BamHI buffer). </li>
@@ Line 18: / Line 16: @@
-<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
+<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4>
-Paweł</h3>
 <p><ol><li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pET15b-OmpA-omega and Z(in GeneArt vector) with NdeI and NotI.</li>
 <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of Z (~200 bp band).</li>