Team:Warsaw/Calendar-Main/19 July 2008

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<h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3>
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<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p>
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
Paweł</h3>
Paweł</h3>

Revision as of 17:40, 11 October 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Result of transformation of pET15b+omega with protein Z DNA: 4 colonies grown
  2. Each colony cultured overnight in LB+amp

Michał L., Ewa, Marcin

We have finally got rid of phage infection and we are able to work with E. coli again. Hurray!


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