Team:Warsaw/Calendar-Main/18 July 2008

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<h3>Cloning of protein A DNA to OmpA constructs</h3>
 
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<li>Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
 
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<li> Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate) inoculated to liquid LB with kanamycin. </li></ol>
 
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
Paweł</h3>
Paweł</h3>

Revision as of 17:45, 11 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Ligation transformed into TOP10 strain and plated on LB+kanamycin

Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin


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