Team:Warsaw/Calendar-Main/18 July 2008
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4> | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4> | ||
- | <p | + | <p>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</p> |
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<h3>Cloning of protein Z DNA to OmpA constructs</h3> | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> |
Revision as of 17:48, 11 October 2008
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPawełLigation transformed into TOP10 strain and plated on LB + ampicillin. Cloning of protein Z DNA to OmpA constructsMichał K.Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin. |