From 2008.igem.org
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| - | <h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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| - | Paweł</h3>
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| - | <p><ol><li>Result of transformation of pET15b+omega with protein Z DNA: 4 colonies grown</li>
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| - | <li>Each colony cultured overnight in LB+amp</li>
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| - | <h3> Michał L., Ewa, Marcin</h3>
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| - | We have finally got rid of phage infection and we are able to work with E. coli again. Hurray!
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Revision as of 17:49, 11 October 2008
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Cloning of protein Z DNA to OmpA constructs
Michał K.
- Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
- Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).
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