Team:Warsaw/Calendar-Main/19 July 2008

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<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li>
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer). We found 4 good clones.</li></ol></p>
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer). We found 4 good clones.</li></ol></p>

Revision as of 17:53, 11 October 2008

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Cloning of protein Z DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha).
  2. Control digest of isolated plasmids with BamHI and SacI (BamHI buffer). We found 4 good clones.

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