Team:Warsaw/Calendar-Main/20 July 2008

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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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Paweł</h3>
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<p><ol><li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inocluated on previous day</li>
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<li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Zero positive results obtained - just empty vectors</li>
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<li>Another overnight <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation>ligation</a> of pET15b-OmpA-omega (NdeI/NotI cutted) with protein Z DNA</li>
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<h3>Cloning omega-A fusion on pKS (second attempt)</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<li>Since the phage is gone we can continue this cloning:</li>
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<li>Restriction digest of PCL product and pKS vector with SacI and NotI</li>
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<li>Ligation of omega-A fusion with pKS vector</li>
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<li>Transformation of Top10 with ligation product</li>
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Latest revision as of 17:58, 11 October 2008

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