Team:Warsaw/Calendar-Main/28 July 2008

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<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one from two highly similar domains.</p>
<p>Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one from two highly similar domains.</p>
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<h3>Checking if omp_omega_A_alpha gives ampicillin resistance<br>
 
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Piotr</h3>
 
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<ol><li>Inoculation of omp_omega_A_alpha into various IPTG concentrations: 0, 0.1, 0.25, 0.5, 0.75, 1 mmol/mL</li></ol>
 

Revision as of 21:01, 11 October 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin:

Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one from two highly similar domains.

Checking the expression of omp_omega_A_alpha and omp_A_alpha
Piotr

  1. Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.

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