Team:Warsaw/Calendar-Main/28 July 2008

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<h3>Checking the expression of omp_omega_A_alpha and omp_A_alpha<br>
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<h3>Checking the expression of omp_omega_A_alpha and omp_A_alpha</h3><h4>Piotr</h4>
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<ol><li>Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.</li></ol>
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<p>Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.</p>

Revision as of 21:20, 11 October 2008

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Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be trunctated and contain only one from two highly similar domains. We inoculated bacteria conatining this plasmid to obtain it for futher cloning.

Checking the expression of omp_omega_A_alpha and omp_A_alpha

Piotr

Inoculation of omp_omega_A_alpha and omp_A_alpha with inductor (0,5 mmol/mL IPTG) and negative control without IPTG.