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Alberta NINT/30 May 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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| + | [[Alberta_NINT/29_May_2008 | < Previous entry]] | [[Alberta_NINT/31_May_2008 | Next entry >]] | ||
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lab work (SD): | lab work (SD): | ||
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Counted colonies on ligation plates: negative plate:0, Positive plate:2 | Counted colonies on ligation plates: negative plate:0, Positive plate:2 | ||
because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow | because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow | ||
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| + | lab work (WM): | ||
| + | Miniprepped 8 O/Ns | ||
| + | Digested K102010/(EcoRI + PstI) | ||
| + | Sequence with VR primer | ||
| + | [[image: NINT_WMpg24b.JPG|500px]] | ||
Latest revision as of 22:12, 11 October 2008
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lab work (SD):
DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep
protocol.
This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
lab work (JD):
Counted colonies on ligation plates: negative plate:0, Positive plate:2
because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
lab work (WM):
Miniprepped 8 O/Ns
Digested K102010/(EcoRI + PstI)
Sequence with VR primer
