Team:Warsaw/Calendar-Main/31 July 2008

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<h3>Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha</h3><h4>Michał K.</h4>
<h3>Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha</h3><h4>Michał K.</h4>
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<p>Separate transformant colonies (tranformations from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/3_August_2008">previous day</a>) inoculated to liquid LB with kanamycin</p>
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<h3>Michał</h3>
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<ol><li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/4_August_2008">previous day</a>. </li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI. </li></ol>
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Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha

Michał K.

Michał

  1. Isolation of plasmids from cultures inocluated on previous day.
  2. Control digest of isolated plasmids with BamHI and SacI.




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