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Alberta NINT/3 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/2_June_2008 | < Previous entry]] | + | [[Alberta_NINT/2_June_2008 | < Previous entry]] | [[Alberta_NINT/4_June_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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Ligated T/A 0 into K102002/B+N and K102004/B+N. | Ligated T/A 0 into K102002/B+N and K102004/B+N. | ||
| - | [[ | + | [[Image:NINT_SD_p25.jpg|750 px]] |
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| + | lab work (WM): | ||
| + | #colonies K102011: 48 negative ctl: 0 | ||
| + | Picked 4 colonies for O/Ns | ||
Latest revision as of 22:16, 11 October 2008
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lab work (SD):
Ran K102002/B+N and K102004/B+N on 2% agarose gel to purify.
Used QIAquick nucleotide removal standard protocol to purify T/A 0.
Ligated T/A 0 into K102002/B+N and K102004/B+N.
lab work (WM):
#colonies K102011: 48 negative ctl: 0
Picked 4 colonies for O/Ns
