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Alberta NINT/13 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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Transformed XL1-B cells with K102007 and negative control | Transformed XL1-B cells with K102007 and negative control | ||
Plated cells and left them for O/N | Plated cells and left them for O/N | ||
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Latest revision as of 22:49, 11 October 2008
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lab work (SD):
Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
cultures, DNA was isolated by QIAprep standard miniprep protocol.
I732018 was sequenced.
I732018 was digested with XbaI + PstI. No evidence of DNA in the gel. Retry!
lab work (JD):
Quantitated K102005/BamH1 and Nco1
Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
Transformed XL1-B cells with K102007 and negative control
Plated cells and left them for O/N
