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Alberta NINT/19 June 2008
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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| + | [[Alberta_NINT/18_June_2008 | < Previous entry]] | [[Alberta_NINT/20_June_2008 | Next entry >]] | ||
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lab work (SD): | lab work (SD): | ||
Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. | Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. | ||
Set up for sequencing. | Set up for sequencing. | ||
| + | |||
| + | lab work (JD): | ||
| + | Counted and recorded number of colonies on newly plated K102007 plates from yesterday | ||
| + | K102007 with the newly recut TA0: 128 | ||
| + | K102007 with heated old TA0: 162 | ||
| + | Negative control: 176 | ||
| + | hence ligation didn't work AGAIN, tomorrow we will try something different and redo ligation on Monday | ||
| + | Mini-prep of 7 O/N colonies of K102003.1-K102003.7 | ||
| + | Set up for sequencing | ||
Latest revision as of 22:52, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol.
Set up for sequencing.
lab work (JD):
Counted and recorded number of colonies on newly plated K102007 plates from yesterday
K102007 with the newly recut TA0: 128
K102007 with heated old TA0: 162
Negative control: 176
hence ligation didn't work AGAIN, tomorrow we will try something different and redo ligation on Monday
Mini-prep of 7 O/N colonies of K102003.1-K102003.7
Set up for sequencing
