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Alberta NINT/23 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/21_June_2008 | < Previous entry]] | + | [[Alberta_NINT/21_June_2008 | < Previous entry]] | [[Alberta_NINT/24_June_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008). | Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008). | ||
| - | + | lab work (JD): | |
| + | Quantitated K102005/B+N | ||
| + | Ligated K102005/B+N with the newly annealed TA0/B+N=K102007 | ||
| + | Transformed XL1-B cells with K102007 | ||
| + | Plated cells on LB+Amp50 | ||
| + | From sequencing K102003.4 becomes official K102003. | ||
Latest revision as of 22:53, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Transformed XL1-B cells with K102002/B+N ligated to TA0/B+N (K102008).
lab work (JD):
Quantitated K102005/B+N
Ligated K102005/B+N with the newly annealed TA0/B+N=K102007
Transformed XL1-B cells with K102007
Plated cells on LB+Amp50
From sequencing K102003.4 becomes official K102003.
