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Alberta NINT/24 June 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/23_June_2008 | < Previous entry]] | + | [[Alberta_NINT/23_June_2008 | < Previous entry]] | [[Alberta_NINT/25_June_2008 | Next entry >]] |
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lab work (SD): | lab work (SD): | ||
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Made overnights of the colonies from 23/06/08 transformation. | Made overnights of the colonies from 23/06/08 transformation. | ||
Made glycerol stock of K102002. | Made glycerol stock of K102002. | ||
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| + | lab work (JD): | ||
| + | Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C | ||
| + | Ran gel slowly on 2% agarose | ||
| + | Cut out desired fragment and gel purified | ||
| + | Counted and recorded colonies from yesterdays ligation: | ||
| + | Negative 200 | ||
| + | K102007 200 | ||
| + | From results it seems ligation didn't work. | ||
| + | Quantified K102003/B+N. | ||
| + | [[Image:annotated4.jpg]] | ||
Latest revision as of 22:53, 11 October 2008
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lab work (SD):
Transformation results: 2 colonies on K102008 plate and 2 colonies on K102008 negative control plate
Redid ligation of K102002/B+N with TA0/B+N (1/100) and transformation into XL1-B cells.
Made overnights of the colonies from 23/06/08 transformation.
Made glycerol stock of K102002.
lab work (JD):
Digested K102003 with BamHI and NcoI and incubated for 2 hrs at 37C
Ran gel slowly on 2% agarose
Cut out desired fragment and gel purified
Counted and recorded colonies from yesterdays ligation:
Negative 200
K102007 200
From results it seems ligation didn't work.
Quantified K102003/B+N.
