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Alberta NINT/8 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/7_July_2008 | < Previous entry]] | + | [[Alberta_NINT/7_July_2008 | < Previous entry]] | [[Alberta_NINT/9_July_2008 | Next entry >]] |
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| + | lab work (SD): | ||
| + | Transformation results: | ||
| + | ara + ara - | ||
| + | K102022 ++++ ++++ | ||
| + | K102032 +++ +++ | ||
| + | K102032 + K102011 - ++ | ||
| + | K102032 + K102034 ++ ++ | ||
| + | K102032 + K102035 - + | ||
| + | K102032 + K102036 + + | ||
| + | |||
| + | where ++++ is the most blue and + is the least blue. - indicates white colonies. | ||
lab work (JD): | lab work (JD): | ||
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Transformed DB3.1 cells with new pSB4A5 part | Transformed DB3.1 cells with new pSB4A5 part | ||
Plated cells on LB +Amp50 plates | Plated cells on LB +Amp50 plates | ||
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Latest revision as of 22:57, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Transformation results:
ara + ara -
K102022 ++++ ++++
K102032 +++ +++
K102032 + K102011 - ++
K102032 + K102034 ++ ++
K102032 + K102035 - +
K102032 + K102036 + +
where ++++ is the most blue and + is the least blue. - indicates white colonies.
lab work (JD):
Collected new quantitative data of K102003/E+P, K102006/E+P and newly cut pSB4A5
Carried out ligation of K102003+pSB4A5 and K102006+pSB4A5
Collected new pSB4A5 from iGEM binder
Transformed XL1-B cells with ligations
Transformed DB3.1 cells with new pSB4A5 part
Plated cells on LB +Amp50 plates
