"
Alberta NINT/16 July 2008
From 2008.igem.org
(Difference between revisions)
m |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/15_July_2008 | < Previous entry]] | + | [[Alberta_NINT/15_July_2008 | < Previous entry]] | [[Alberta_NINT/17_July_2008 | Next entry >]] |
| + | |||
lab work (SD): | lab work (SD): | ||
| Line 22: | Line 23: | ||
Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P | Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P | ||
Ran gel electrophoresis, 2% gel, 100V | Ran gel electrophoresis, 2% gel, 100V | ||
| - | [[Image:UVP00326 | + | [[Image:UVP00326.jpg]] |
| - | + | ||
| - | + | ||
Latest revision as of 23:00, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Since the double-plasmid cell cultures failed to grow on the LB+amp50+kan35 plates we will try to
grow them on LB+kan35. Each of the plasmids is also transformed alone into XL1-B cells and plated
on kan35 plates as well.
K102022 (LB+amp50)
K102032 (LB+amp50)
K102032+K102011 (LB+kan35)
K102032+K102034 (LB+kan35)
K102032+K102035 (LB+kan35)
K102032+K102036 (LB+kan35)
K102011 (LB+kan35)
K102034 (LB+kan35)
K102035 (LB+kan35)
K102036 (LB+kan35)
lab work (JD):
Mini-prep of pSB4A5, K102003, K102006
Restriction digests: pSB4A5/E+P, K102003/E+P and K102006/E+P
Ran gel electrophoresis, 2% gel, 100V
