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Team:Warsaw/Calendar-Main/18 June 2008
From 2008.igem.org
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pACYC177.</li> | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of pACYC177.</li> | ||
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI (Tango 2x buffer). </li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of purified pACYC177 with NdeI and BamHI (Tango 2x buffer). </li> | ||
| - | <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">isolation</a> of proper band (3200 bp).</li> | + | <li> Gel electrophoresis (<a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_June_2008#fig2">Fig. 2</a>) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">isolation</a> of proper band (3200 bp).</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products (<a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_June_2008#fig1">Fig. 1</a>). </li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Clean-up</a> of OmpA_alpha and OmpA_omega digest products (<a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/18_June_2008#fig1">Fig. 1</a>). </li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified DNA (pACYC177 with OmpA_alpha and OmpA_omega DNA fragments).</li> | ||
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<a name="fig1"> <img src="https://static.igem.org/mediawiki/2008/c/c9/Ompa_Alpha_Omega_Cleanup_WAW.jpg" width=300/> </a> | <a name="fig1"> <img src="https://static.igem.org/mediawiki/2008/c/c9/Ompa_Alpha_Omega_Cleanup_WAW.jpg" width=300/> </a> | ||
<var> Fig. 1. OmpA_alpha (lane 2) and OmpA_omega (lane 3) digest products.</var> | <var> Fig. 1. OmpA_alpha (lane 2) and OmpA_omega (lane 3) digest products.</var> | ||
| + | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/5/5f/PACYC177_digest_WAW.jpg" width=300/></a> | ||
| + | <var>Fig. 2. pACYC177 digested with NdeI and BamHI (lane 2).</var> | ||
<h3> Blue/white and rifampicin test </h3> | <h3> Blue/white and rifampicin test </h3> | ||
Revision as of 01:04, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.
Fig. 1. OmpA_alpha (lane 2) and OmpA_omega (lane 3) digest products.
Fig. 2. pACYC177 digested with NdeI and BamHI (lane 2).
Blue/white and rifampicin testMichał L., Ewa, MarcinCounting of blue colonies:
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