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Team:Warsaw/Calendar-Main/24 June 2008
From 2008.igem.org
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primers.</li> | primers.</li> | ||
| - | <li>Gel electrophoresis (no proper clones founded).</li> | + | <li>Gel electrophoresis (Fig. 1, no proper clones founded).</li> |
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<p>Sequencing determined that all white colonies from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a> have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p> | <p>Sequencing determined that all white colonies from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_June_2008">16 June</a> have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ).</p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=400/> | ||
| + | <var>Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var> | ||
Revision as of 01:27, 12 October 2008
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Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinStill no success. We need to run gradient PCR to find optimal reaction conditions. Preparation of constructs with OmpA protein fusionsMichał K.
Sequencing determined that all white colonies from 16 June have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ). 
Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.
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