Team:Chiba/jk/γ/trs/0829

From 2008.igem.org

(Difference between revisions)
(→29,September,2008)
(→29,September,2008)
 
(4 intermediate revisions not shown)
Line 5: Line 5:
:LacZ
:LacZ
:Venus YFP(pGEX)
:Venus YFP(pGEX)
 +
*試薬濃度
 +
:IPTG--->
 +
:X-gal-->20μl
*Result
*Result
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
[[Image:Solid0829.gif]]
-
<tr>
+
*考察
-
<td width="257">Sample No</td>
+
:LacZの発現が予想よりも遅くなった。->X-galの濃度が薄すぎて発現が遅かった？->濃度を高くして実験。
-
<td>pGFP</td><td>sfGFP</td><td>LacZ</td><td>Venus YFP</td>
+
-
</tr>
+
-
<tr>
+
-
<td>0min</td>
+
-
<td>-</td><td>+</td><td>-</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>30min</td>
+
-
<td>-</td><td>+</td><td>-</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>60min</td>
+
-
<td>-</td><td>+</td><td>-</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>120min</td>
+
-
<td>O</td><td>0.5</td><td>0.5</td><td>0.5</td><td>0</td><td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA(x10)</td>
+
-
<td>0.6</td><td>7</td><td>7</td><td>6</td><td>2</td><td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer2</td>
+
-
<td>6</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer3</td>
+
-
<td>-</td><td>7</td><td>7</td><td>6</td><td>-</td><td>7<td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>1.9</td><td>1</td><td>1</td><td>2</td><td>0.5</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>60</td><td>70</td><td>70</td><td>60</td><td>20</td><td>70</td>
+
-
</tr>
+
-
</table>
+
 +
*X-gal濃度変更
 +
:40μl
 +
:80μl
 +
 +
*Result
 +
[[Image:Solid0830Xgal.gif]]
Line 55: Line 25:
<br>>[[Team:Chiba/jk/γ/trs|TIME RESPONCE (solid)]]
<br>>[[Team:Chiba/jk/γ/trs|TIME RESPONCE (solid)]]
<br>>[[Team:Chiba/jk/γ|出力班]]
<br>>[[Team:Chiba/jk/γ|出力班]]
 +
<br>>[[Team:Chiba/jk|実験ログ]]
 +
 +
 +
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
 +
!align="center"|[[Team:Chiba/j|ホーム]]
 +
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
 +
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
 +
!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
 +
!align="center"|[[Team:Chiba/Internal|ノート]]
 +
|}

Latest revision as of 06:21, 12 October 2008

29,September,2008

  • 計測対象
pGFP
sfGFP
LacZ
Venus YFP(pGEX)
  • 試薬濃度
IPTG--->
X-gal-->20μl
  • Result

Solid0829.gif

  • 考察
LacZの発現が予想よりも遅くなった。->X-galの濃度が薄すぎて発現が遅かった？->濃度を高くして実験。
  • X-gal濃度変更
40μl
80μl
  • Result

Solid0830Xgal.gif



>TIME RESPONCE (solid)
>出力班
>実験ログ


ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B3/trs/0829"