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Team:Warsaw/Calendar-Main/23 June 2008
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<h3>Preparation of alpa-A and omega-A fusions</h3> | <h3>Preparation of alpa-A and omega-A fusions</h3> | ||
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
| - | <p>We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> | + | <p>We have successfully amplified Omega-linker (Fig. 1.), but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p> |
| + | <img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/> | ||
| + | <var><b>Fig. 1.</b>PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.</var> | ||
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Revision as of 10:56, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpa-A and omega-A fusionsMichał L., Ewa, MarcinWe have successfully amplified Omega-linker (Fig. 1.), but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
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