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Team:Warsaw/Calendar-Main/24 June 2008
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primers.</li> | primers.</li> | ||
| - | <li>Gel electrophoresis (Fig. 1, no proper clones | + | <li>Gel electrophoresis (<a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/Calendar-Main/24_June_2008#fig1">Fig. 1</a>, no proper clones found).</li> |
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| - | <img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=400/> | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=400/></a> |
| - | <var>Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var> | + | <var><b>Fig. 1.</b> PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var> |
Revision as of 11:02, 12 October 2008
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Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinStill no success. We need to run gradient PCR to find optimal reaction conditions. Preparation of constructs with OmpA protein fusionsMichał K.
Sequencing determined that all white colonies from 16 June have intact lacZ gene. Reason of white phenotype unknown. We suppose that it is due to chromosomal mutation. We need another reporter system (not splitted to chromosomal and plasmid part --> GFP? ). 
Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.
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